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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
05/11/2014 |
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Data da última atualização: |
26/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MONDIN, M.; SEREJO, J. A. dos S.; BERTÃO, M. R.; LABORDA, P.; PIZZAIA, D.; AGUIAR-PERECIN, M. L. R. |
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Afiliação: |
MATEUS MONDIN, ESALQ; JANAY ALMEIDA DOS SANTOS SEREJO, CNPMF; MÔNICA R. BERTÃO, ESALQ; PRIANDA LABORDA, UNICAMP; DANIEL PIZZAIA, ESALQ; MARGARIDA L. R. AGUIAR-PERECIN, ESALQ. |
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Título: |
Karyotype variability in tropical maize sister inbred lines and hybrids compared with KYS standard line. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Frontiers in Plant Science, October, v. 5, 2014. |
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ISSN: |
1664-462X |
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Idioma: |
Inglês |
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Conteúdo: |
Maize karyotype variability has been extensively investigated. The identification of maize somatic and pachytene chromosomes has improved with the development of fluorescence in situ hybridization (FISH) using tandemly repeated DNA sequences as probes. We identified the somatic chromosomes of sister inbred lines that were derived from a tropical flint maize population (Jac Duro [JD]), and hybrids between them, using FISH probes for the 180-bp knob repeat, centromeric satellite (CentC), centromeric satellite 4 (Cent4), subtelomeric clone 4-12-1, 5S ribosomal DNA and nucleolus organizing region DNA sequences. The observations were integrated with data based on C-banded mitotic metaphases and conventional analysis of pachytene chromosomes. Heterochromatic knobs visible at pachynema were coincident with C-bands and 180-bp FISH signals on somatic chromosomes, and most of them were large. Variation in the presence of some knobs was observed among lines. Small 180-bp knob signals were invariant on the short arms of chromosomes 1, 6, and 9. The subtelomeric 4-12-1 signal was also invariant and useful for identifying some chromosomes. The centromere location of chromosomes 2 and 4 differed from previous reports on standard maize lines. Somatic chromosomes of a JD line and the commonly used KYS line were compared by FISH in a hybrid of these lines. The pairing behavior of chromosomes 2 and 4 at pachytene stage in this hybrid was investigated using FISH with chromosome-specific probes. The homologues were fully synapsed, including the 5S rDNA and CentC sites on chromosome 2, and Cent4 and subtelomeric 4-12-1 sites on chromosome 4. This suggests that homologous chromosomes could pair through differential degrees of chromatin packaging in homologous arms differing in size. The results contribute to current knowledge of maize global diversity and also raise questions concerning the meiotic pairing of homologous chromosomes possibly differing in their amounts of repetitive DNA. MenosMaize karyotype variability has been extensively investigated. The identification of maize somatic and pachytene chromosomes has improved with the development of fluorescence in situ hybridization (FISH) using tandemly repeated DNA sequences as probes. We identified the somatic chromosomes of sister inbred lines that were derived from a tropical flint maize population (Jac Duro [JD]), and hybrids between them, using FISH probes for the 180-bp knob repeat, centromeric satellite (CentC), centromeric satellite 4 (Cent4), subtelomeric clone 4-12-1, 5S ribosomal DNA and nucleolus organizing region DNA sequences. The observations were integrated with data based on C-banded mitotic metaphases and conventional analysis of pachytene chromosomes. Heterochromatic knobs visible at pachynema were coincident with C-bands and 180-bp FISH signals on somatic chromosomes, and most of them were large. Variation in the presence of some knobs was observed among lines. Small 180-bp knob signals were invariant on the short arms of chromosomes 1, 6, and 9. The subtelomeric 4-12-1 signal was also invariant and useful for identifying some chromosomes. The centromere location of chromosomes 2 and 4 differed from previous reports on standard maize lines. Somatic chromosomes of a JD line and the commonly used KYS line were compared by FISH in a hybrid of these lines. The pairing behavior of chromosomes 2 and 4 at pachytene stage in this hybrid was investigated using FISH with chromosome-specific probes.... Mostrar Tudo |
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Palavras-Chave: |
Heterochromatic knobs. |
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Thesagro: |
Milho. |
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Thesaurus Nal: |
Centromeres; Chromosomes; Corn; Satellite DNA. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02751naa a2200265 a 4500
001 1999223
005 2023-05-26
008 2014 bl uuuu u00u1 u #d
022 $a1664-462X
100 1 $aMONDIN, M.
245 $aKaryotype variability in tropical maize sister inbred lines and hybrids compared with KYS standard line.$h[electronic resource]
260 $c2014
520 $aMaize karyotype variability has been extensively investigated. The identification of maize somatic and pachytene chromosomes has improved with the development of fluorescence in situ hybridization (FISH) using tandemly repeated DNA sequences as probes. We identified the somatic chromosomes of sister inbred lines that were derived from a tropical flint maize population (Jac Duro [JD]), and hybrids between them, using FISH probes for the 180-bp knob repeat, centromeric satellite (CentC), centromeric satellite 4 (Cent4), subtelomeric clone 4-12-1, 5S ribosomal DNA and nucleolus organizing region DNA sequences. The observations were integrated with data based on C-banded mitotic metaphases and conventional analysis of pachytene chromosomes. Heterochromatic knobs visible at pachynema were coincident with C-bands and 180-bp FISH signals on somatic chromosomes, and most of them were large. Variation in the presence of some knobs was observed among lines. Small 180-bp knob signals were invariant on the short arms of chromosomes 1, 6, and 9. The subtelomeric 4-12-1 signal was also invariant and useful for identifying some chromosomes. The centromere location of chromosomes 2 and 4 differed from previous reports on standard maize lines. Somatic chromosomes of a JD line and the commonly used KYS line were compared by FISH in a hybrid of these lines. The pairing behavior of chromosomes 2 and 4 at pachytene stage in this hybrid was investigated using FISH with chromosome-specific probes. The homologues were fully synapsed, including the 5S rDNA and CentC sites on chromosome 2, and Cent4 and subtelomeric 4-12-1 sites on chromosome 4. This suggests that homologous chromosomes could pair through differential degrees of chromatin packaging in homologous arms differing in size. The results contribute to current knowledge of maize global diversity and also raise questions concerning the meiotic pairing of homologous chromosomes possibly differing in their amounts of repetitive DNA.
650 $aCentromeres
650 $aChromosomes
650 $aCorn
650 $aSatellite DNA
650 $aMilho
653 $aHeterochromatic knobs
700 1 $aSEREJO, J. A. dos S.
700 1 $aBERTÃO, M. R.
700 1 $aLABORDA, P.
700 1 $aPIZZAIA, D.
700 1 $aAGUIAR-PERECIN, M. L. R.
773 $tFrontiers in Plant Science, October$gv. 5, 2014.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
12/12/2018 |
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Data da última atualização: |
04/12/2019 |
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Tipo da produção científica: |
Documentos |
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Autoria: |
RIBEIRO, J. F.; PERES, M. K.; SANTOS, D. da S.; SAMPAIO, A. B.; OGATA, R. S.; SOUZA, R. M.; OLIVEIRA, M. C.; DURIGAN, G.; SILVA JÚNIOR, M. C. da; MUNHOZ, C. B. R.; VALLS, J. F. M.; NEHME, L.; BIANCHETTI, L. de B.; BRINGEL JÚNIOR, J. B. de A.; WALTER, B. M. T. |
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Afiliação: |
JOSE FELIPE RIBEIRO, CPAC; JOSE FRANCISCO MONTENEGRO VALLS, Cenargen; LUCIANO DE BEM BIANCHETTI, Cenargen; BRUNO MACHADO TELES WALTER, Cenargen. |
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Título: |
Espécies vegetais nativas recomendadas para recomposição ambiental no bioma Cerrado. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Planaltina, DF: Embrapa Cerrados, 2018. |
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Páginas: |
49 p. |
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Idioma: |
Português |
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Thesagro: |
Cerrado; Reflorestamento; Regeneração; Vegetação Nativa. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00912nam a2200325 a 4500
001 2101530
005 2019-12-04
008 2018 bl uuuu u0uu1 u #d
100 1 $aRIBEIRO, J. F.
245 $aEspécies vegetais nativas recomendadas para recomposição ambiental no bioma Cerrado.
260 $aPlanaltina, DF: Embrapa Cerrados$c2018
300 $a49 p.
650 $aCerrado
650 $aReflorestamento
650 $aRegeneração
650 $aVegetação Nativa
700 1 $aPERES, M. K.
700 1 $aSANTOS, D. da S.
700 1 $aSAMPAIO, A. B.
700 1 $aOGATA, R. S.
700 1 $aSOUZA, R. M.
700 1 $aOLIVEIRA, M. C.
700 1 $aDURIGAN, G.
700 1 $aSILVA JÚNIOR, M. C. da
700 1 $aMUNHOZ, C. B. R.
700 1 $aVALLS, J. F. M.
700 1 $aNEHME, L.
700 1 $aBIANCHETTI, L. de B.
700 1 $aBRINGEL JÚNIOR, J. B. de A.
700 1 $aWALTER, B. M. T.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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